ESTIMATION OF ANTIBACTERIAL POTENTIAL OF LEAF EXTRACT OF PORTULACA GRANDIFLORA HOOK. AND ALSTONIA SCHOLARIS L.
Keywords:
bacteria, contamination, MBC, leaves extract,, MIC, S. aureusAbstract
Recent investigations have clearly shown that pathogenic bacteria are quickly increasing in quantity and are the root of numerous severe diseases. Equipment resembling brush sonication and alcohol wipes had been utilized for cleaning these pathogens. In the current study, P. grandiflora and A. scholaris leaves extract were used to reduce surface contamination. It had been discovered that plant leaf extracts have antibacterial qualities and can improve surface cleaning. P. grandiflora and A. scholaris plant leaves were gathered, as well as bacterial samples from the four different locations that are, washroom flush, sink, floor and door knob. Bacteria were cultivated independently for each of the four sites and the streak plate approach was used to culture them. Bacteria were studied microscopically by using gram staining technique. Under an optical microscope (Olympus CX23), the two bacteria S. aureus and E. coli were discovered. MIC technique was done to determine the concentration (of our leaf extracts) at which bacteria were eliminated or decreased. The results revealed that the MIC value against bacteria (S. aureus) decreased with increase in concentration of leaf extracts. MBC of both leaf extracts were also calculated. According to this study, plant extracts are able to improve surface cleaning. They can be used into soaps or detergents to enhance their cleaning capabilities.