In vitro production of novel volatile compounds from Foeniculum vulgare Mill. cell lines

Abstract

The idea to get desired quality and quantity of volatile compounds by altering the amount and concentration of PGRs during in vitro growth of a plant is considered as one of the most advanced ones, in modern bioscience technologies. During the present piece of work, callogenesis (81%), somatic embryogenesis (92%) and regeneration (90%) on MS basal medium, supplemented with 2, 4-D (3.0mg/L) from Foeniculum vulgare Mill.’s leaf explants. Identification of volatile compounds during in vitro cell lines growth of fennel was done using Gas Chromatography Mass Spectrophotometer (GC/MS). Sixty five compounds were identified during callogenesis, out of which two were found to be novel. One hundred and twenty compounds were detected during somatic embryogenesis, out of which nine were novel. Similarly, one hundred compounds were found during regeneration out of which three were identified as the novel ones. It is noteworthy that above mentioned experimental technique to produce desired quantity and quality of novel/already cited  compounds by manipulating various PGRs during in vitro growth of a plant, can be  utilized in future for commercial purposes on a larger scale  in pharmaceutical industry.